Job Description
Duties & Responsibilities:
- Perform sandwich ELISA assays for RNA synthetase and RNA polymerase lysates.
- Conduct Bradford total protein assays on raw material lysates and in-process samples.
- Perform GFP activity assays, Capillary Western (Jess/Leo), and A280/SoloVPE analysis.
- Support analytical method qualification, including accuracy, repeatability, and intermediate precision.
- Qualify and manage critical reagents, antibodies, reference standards, and assay controls.
- Maintain accurate experimental records using Benchling ELN.
- Troubleshoot analytical methods, instrumentation, and assay variability.
- Support high-throughput automated ELISA using Tecan EVO and robotic liquid-handling systems.
- Analyze experimental data, present findings, and collaborate with cross-functional teams.
- Support method development, process characterization, and training of backup analysts.
Required Experience & Skills:
- 10+ years of hands-on laboratory experience.
- Strong experience in ELISA and Bradford protein assays.
- Expertise in protein quantification and analytical characterization.
- Knowledge of Capillary Western, GFP activity assays, and A280/SoloVPE.
- Experience with method qualification and laboratory automation is preferred.
- Strong troubleshooting, data analysis, and documentation skills