Scientist – I

Job Description

Education and Experience:

• Bachelor’s Degree/ Master’s Degree in life science related fields with 1-2 years of lab experience in academic or industry(preferred).

Essential Skills:

1. Biochemical Assays:

• Possesses a foundational understanding of biochemical assay workflows, including tissue homogenization techniques.
• Highly detail-oriented and demonstrates the ability to quickly learn and execute new protocols.
• Maintains accurate and precise records using electronic lab notebooks (e.g., Signals) and adapts readily to evolving experimental needs.

2. Lab Safety & Cleanliness:

• Follows established biosafety protocols and decontamination procedures for shared lab equipment.
• Ability and willingness to work with BSL-2 derived human or rodent material.
• Maintains a clean and safe working environment and ensures shared equipment is properly maintained and repaired as needed.

3. GLP Compliance:

• Adheres strictly to Good Laboratory Practice (GLP) standards, ensuring accurate documentation, traceability, and preservation of sample integrity.

4. Communication & Collaboration:

• Proficient in Microsoft Office for data handling and team communication.
• Collaborates effectively with scientists to meet experimental timelines and assay requirements.

5. Work Commitment:

• This is an in-person, on-site role with an expectation of approximately 80% in-lab work.

6. Tissue Processing:

• Skilled in tissue homogenization using manual (e.g., cryopulverization, Dounce) and mechanical (e.g., Qiagen Tissue Lyser, Bioruptor, sonication) methods.
• Familiar with detergent-based extraction techniques (e.g., RIPA, Triton X-100, Sarkosyl, guanidine hydrochloride).

7. Sample Handling:

• Capable of accurately labeling and processing large volumes of BSL-2 level samples.
• Demonstrates precision in manual pipetting and transferring of homogenates/biofluids, with experience or willingness to work with human and rodent-derived materials.

8. Protein Quantification:

• Familiar with or able to quickly learn BCA protein assays, including the Pierce Dilution-Free Rapid Gold BCA method.
• Uses instruments such as the client for data capture and GraphPad Prism for analysis.
• Prepares samples for downstream analysis and communicates effectively to meet program deliverables.

9. Equipment Operation:

• Experienced with or has the aptitude to learn operation of lab equipment such as homogenizers, sonicators, ultracentrifuges, and the client.