Downstream Purification Scientist

Job Description

Job Summary:

• We are seeking a highly motivated Contractor – Purification Scientist to join our Downstream Process Development team.
• The successful candidate will contribute to the purification and characterization of viral vectors, biologics, or recombinant proteins to support process development and manufacturing activities.
• The ideal candidate has hands-on experience in Cesium chloride (CsCl) density gradient ultracentrifugation, ion-exchange chromatography, affinity purification, and tangential flow filtration (TFF), with a strong understanding of purification principles and data analysis.

Duties and Responsibilities:

• Execute purification processes including CsCl density gradient ultracentrifugation, affinity chromatography (e.g., AVB, Capture Select), ion-exchange chromatography (AEX, CEX), and other polishing steps.
• Operate AKTA systems (Avant, Pure, or Pilot) for small- to large-scale purification runs.
• Perform TFF operations for buffer exchange and concentration of intermediate or final products.
• Support experimental design, data collection, and documentation following established procedures and GMP/GLP best practices.
• Assist in troubleshooting and optimization of purification processes.
• Analyze in-process and purified samples using relevant analytical methods (e.g., UV, SDS-PAGE, ddPCR, ELISA, or HPLC).
• Collaborate cross-functionally with upstream, analytical, and manufacturing teams.
• Maintain lab equipment, manage buffers and column inventory, and ensure compliance with safety and documentation standards.

Education:

• Master’s degree in biology, biochemistry, chemical engineering, biotechnology, or related field.

Experience:

• Minimum 2 years of hands-on purification experience in an academic or industry setting.

Technical Skills:

• Strong knowledge and practical experience with CsCl density gradient ultracentrifugation, ion-exchange, and affinity chromatography.
• Familiarity with buffer preparation.
• Understanding of basic analytical methods for product characterization.

Soft Skills:

• Strong attention to detail and excellent documentation habits.
• Effective communication and teamwork skills.
• Ability to manage multiple tasks and meet project timelines.

Preferred Qualifications:

• Experience in AAV or other viral vector CsCl density gradient and column purification.
• Experience with AKTA systems and Unicorn software.