Cell Line Development Research Associate

Job Description

Job Summary:

• Cell Line Development Research Associate is involved in the creation of stable cell lines for the recombinant adeno-associated viral vector (rAAV) production processes.
• He/she executes experiments, produces relevant documentation, and works under supervision.

Duties and Responsibilities:

• Culturing mammalian cells for viral production in shake flasks, spinner flasks, spin tubes, deep well and automation platforms.
• Performing experiments in the development, optimization and scale-up of AAV producer cell lines.
• Preparing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.
• Optimization of transfection and transduction protocols for newly engineered cell lines.
• Maintaining an accurate laboratory notebook.
• Ensuring work conforms to applicable quality and safety standards.
• Participating on project teams (as needed).

Education and Experience:

• B.S. with 3-6 years of experience, M.Sc. with 2-5 years of relevant scientific or technical experience in the life sciences industry or equivalent.
• Experience in cell culture including materials, instruments and technologies is essential.
• Experience with viral vector production is preferred.
• Hands-on experience in mammalian cell culture production processes is a critical requirement.
• Experience with creation, analysis and characterization of new engineered cell lines and stability studies (subcloning, clonal expansion, transient transfection and expression screening) is a strong plus.
• Experience in molecular biology techniques (plasmid design, molecular cloning, SDS-PAGE/western blotting, qPCR, etc.) is a strong plus.
• Experience in laboratory automation hardware and software is a plus.

Knowledge, Skills and Abilities:

• This includes small scale (shaker flasks, spinner flasks, spin tubes, deep well) and/or benchtop bioreactor scale.
• Automation and ELN experience is a strong plus.
• Effective communication skills (verbal, written, interpersonal).
• Industry experience is a plus but not required.
• Basic Knowledge on AAV biology is a plus.
• Must be able to interact with cross-functional teams.
• Analytical and problem-solving skills.
• Ability to manage time effectively.
• Teamwork is a must.

Physical Requirements:

• Must be able to lift 25 pounds.
• Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed.
• On occasion, long work hours may be required depending upon the experiment schedule.
• Handling large volumes of cell culture or related materials, such as tens of liters, may be required.
• Also, several viral materials, including AAV may be involved in the work.

Interview:

• First Round Virtual.
• 2nd Round Panel Interview Virtual.