Research Assistant II

Job Description

Duties and Responsibilities:

• Support expression, conjugation, purification and characterization of proteins.
• Develop and conduct biophysical and functional assays to validate various protein designs.
• Perform plate-based assays to analyze samples downstream from in vivo studies.
• Analyze, integrate and interpret data from multiple sources.
• Diligently document work in digital systems and regularly communicate results in team and group settings.
• Prepare reagents, solutions, and samples for analysis, including samples collected from in vivo studies (e.g. tissue homogenization and protein extraction).
• Assist in method development, validation, and troubleshooting.
• Ensure compliance with safety regulations and good research practices.
• Work collaboratively in a team environment and manage priorities to achieve our goals.

Education and Experience:

• Bachelor’s or Master’s degree in Protein Biochemistry/Biochemistry preferred, or related field.
• With a Bachelor’s Degree – At least 3-5 years of hands-on laboratory experience (industry).
• With a Master’s Degree – At least 1–3 years of hands-on laboratory experience (industry).
• Experience with recombinant protein production, purification, and characterization.
• Experience in FPLC protein purification using AKTA systems and Unicorn software (e.g. affinity chromatography, SEC, and IEX).
• Experience in plate based functional assays (ELISA/client, functional assays, enzyme kinetics, chromogenic/fluorescent readouts, etc.)
• Experience with protein bioconjugation and conjugate purification.
• Experience with OligonucleoProduct and related methods a plus.
• Experience using Benchling (eLN).

Knowledge, Skills and Abilities:

• Protein characterization techniques (e.g. SDS-PAGE, Western Blots, endotoxin testing, HPLC, BLI (Octet) or SPR (Biacore)).
• Strong organizational and documentation skills.
• Ability to work independently and as part of a team.
• Excellent communication and problem-solving skills.
• A creative, problem-solving, collaborative, and goal-oriented mindset.