Molecular and Cellular Biologist

Job Description

Job Summary:

• We are seeking a scientist to join our Systems Immunology Lab in client’s Inflammation & Immunology Research Unit.
• The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team.

Duties and Responsibilities:

• Generate custom lentiviral libraries and maintain extensive cell cultures.
• Generate edited pools of cells via lentiviral transduction and arrayed electroporation.
• Generate single-cell data via several commercial workflows, including from 10x Genomics.
• Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale.

Education and Experience:

• Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field.
• 3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry.

Hard Skills:

• Diverse Cell Types.
• Molecular biology techniques *qPCR.
• Gene Editing.

Preferred:

• Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.

Physical Requirements:

• Typical bench work, including sitting, pipetting, lifting <10lb items.

Interview Process:

• 1st Teams screen (Camera-On).
• 2nd Onsite (2hr with Stakeholders).